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addgene pcfj601  (Addgene inc)


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    Structured Review

    Addgene inc addgene pcfj601
    Addgene Pcfj601, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcfj601+addgene/pCFJ601+-+Peft-3+Mos1+transposase+(Plasmid+%2334874)/pm40624357-274-0-0
    Average 93 stars, based on 39 article reviews
    addgene pcfj601 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Sequencing:

    Article Title: Evolution of Yin and Yang isoforms of a chromatin remodeling subunit precedes the creation of two genes
    Article Snippet: DOI: https://doi.org/10.7554/eLife.48119 22 of 38 Continued Reagent type or resource Designation Source of reference Identifiers Additional information Strain (C. elegans) PTM416 This paper RRID: WB-STRAIN:PTM416 Strain Background: N2, Request a strain: please email the corresponding author Strain (C. elegans) PTM417 This paper RRID: WB-STRAIN:PTM417 Strain Background: N2, Request a strain: please email the corresponding author Strain (C. elegans) PTM420 This paper RRID: WB-STRAIN:PTM420 Strain Background: N2, Request a strain: please email the corresponding author Strain (C. elegans) PTM487 This paper RRID: WB-STRAIN:PTM487 Strain Background: N2, Request a strain: please email the corresponding author Strain (C. elegans) PTM489 This paper RRID: WB-STRAIN:PTM489 Strain Background: N2, Request a strain: please email the corresponding author Strain (C. elegans) PTM512 This paper RRID: WB-STRAIN:PTM512 Strain Background: N2, Request a strain: please email the corresponding author Strain (C. elegans) PTM517 This paper RRID: WB-STRAIN:PTM517 Strain Background: N2, Request a strain: please email the corresponding author Cell line (Human) Colo-205 American Type Culture Collection (Rockville, MD) Cell line (Human) MCF-7 American Type Culture Collection (Rockville, MD) Cell line (Human) MDA-MB-231 American Type Culture Collection (Rockville, MD) Cell line (Human) Hela American Type Culture Collection (Rockville, MD) Cell line (Human) A549 G. Roncador, CNIO Sequence-based reagents (Plasmid) Plasmid: pSM Cori Bargmann Lab (Rockefeller University) Sequence-based reagents (Plasmid) Plasmid: pDD162 PrU6::dpy-10_sgRNA PMID: 27467070 CRISPR/Cas9 gene editing Sequence-based reagents (Plasmid) Plasmid: pDD162 Preft3::Cas9 PMID: 27467070 CRISPR/Cas9 gene editing Sequence-based reagents (Plasmid) Plasmid: pCFJ90 Addgene http://www.wormbuilder.org /test-page/about-mossci/ Sequence-based reagents (Plasmid) Plasmid: pCFJ104 Addgene http://www.wormbuilder.org /test-page/about-mossci/ Sequence-based reagents (Plasmid) Plasmid: pCFJ151 Addgene http://www.wormbuilder.org /test-page/about-mossci/ Continued on next page Xu et al. eLife 2019;8:e48119. .. DOI: https://doi.org/10.7554/eLife.48119 23 of 38 Continued Reagent type or resource Designation Source of reference Identifiers Additional information Sequence-based reagents (Plasmid) Plasmid: pCFJ601 Addgene http://www.wormbuilder.org /test-page/about-mossci/ Antibody (mouse monoclonal) anti-HA Life Technologies Cat. No.: 326700 (1:500) Antibody (mouse monoclonal) anti-DYKDDDDK Life Technologies Cat. No.: MA191878 (1:1000) Antibody (mouse monoclonal) anti-FLAG Millipore Sigma Cat. No.: F3165 (1:1000) Antibody Horseradish peroxidaseconjugated secondary antibodies Dako Glostrup (1:10000) Peptide, recombinant protein BPTF Novus Biologicals Cat. No.: NB100-41418 Peptide, recombinant protein Vinculin Sigma Cat. No.: V9131 Sequence-based reagents (Oligonucleotide) dpy-10 (cn64) PMID: 25161212 CRISPR/Cas9 gene editing Commercial assay, kit Taqman probe: dpy-10 (kah82/kah83) Thermal: Custom TaqMan SNP Genotyping Assays PTM09 Commercial assay, kit NEB Q5 site directed mutagenesis kit NEB Cat. No.: E0554 Commercial assay, kit Next Ultra II Directional RNA Library Prep Kit NEB Cat. No.: E7760S Commercial assay, kit Zymo DNA isolation kit Zymo Cat. No.: D4071 Commercial assay, kit Zymo DNA cleanup kit Zymo Cat. No.: D4064 Commercial assay, kit ddPCR Supermix for Probes BIORAD Cat. No.: 1863010 Commercial assay, kit Droplet Generation Oils BIORAD Cat. No.: 1863005 Commercial assay, kit ddPCR Droplet Reader Oil BIORAD Cat. No.: 1863004 Commercial assay, kit VECTASHIELD antifade Mounting Medium with DAPI VECTOR Cat. No.: H-1200 Software, Algorithm edgeR PMID: 19910308 RRID:SCR_012802 Opensource: https://bioconductor.org/ packages/release/bioc /html/edgeR.html Software, Algorithm SARtools PMID: 27280887 RRID:SCR_016533 Opensource: https://github.com/ PF2-pasteur-fr/SARTools Software, Algorithm IGV PMID: 21221095 RRID:SCR_011793 https://software. broadinstitute.org/ software/igv/ Software, Algorithm Kallisto PMID: 27043002 RRID:SCR_016582 https://pachterlab. github.io/kallisto/ Software, Algorithm HISAT2 PMID: 25751142 RRID:SCR_015530 https://ccb.jhu.edu/ software/hisat2/index.shtml Continued on next page Xu et al. eLife 2019;8:e48119. .. DOI: https://doi.org/10.7554/eLife.48119 24 of 38 Continued Reagent type or resource Designation Source of reference Identifiers Additional information Software, Algorithm Samtools PMID: 19505943 RRID:SCR_002105 http://samtools. sourceforge.net/ Software, Algorithm Jalview PMID: 19151095 RRID:SCR_006459 http://www.jalview.org/ Software, Algorithm MAFFT PMID: 23329690 RRID:SCR_011811 https://mafft.cbrc.jp/ alignment/software/ Software, Algorithm IQ-Tree PMID: 25371430 RRID:SCR_017254 http://www.iqtree.org Software, Algorithm ITOL PMID: 27095192 https://itol.embl.de/ Software, Algorithm PAL2NAL PMID: 16845082

    Plasmid Preparation:

    Article Title: Evolution of Yin and Yang isoforms of a chromatin remodeling subunit precedes the creation of two genes
    Article Snippet: DOI: https://doi.org/10.7554/eLife.48119 22 of 38 Continued Reagent type or resource Designation Source of reference Identifiers Additional information Strain (C. elegans) PTM416 This paper RRID: WB-STRAIN:PTM416 Strain Background: N2, Request a strain: please email the corresponding author Strain (C. elegans) PTM417 This paper RRID: WB-STRAIN:PTM417 Strain Background: N2, Request a strain: please email the corresponding author Strain (C. elegans) PTM420 This paper RRID: WB-STRAIN:PTM420 Strain Background: N2, Request a strain: please email the corresponding author Strain (C. elegans) PTM487 This paper RRID: WB-STRAIN:PTM487 Strain Background: N2, Request a strain: please email the corresponding author Strain (C. elegans) PTM489 This paper RRID: WB-STRAIN:PTM489 Strain Background: N2, Request a strain: please email the corresponding author Strain (C. elegans) PTM512 This paper RRID: WB-STRAIN:PTM512 Strain Background: N2, Request a strain: please email the corresponding author Strain (C. elegans) PTM517 This paper RRID: WB-STRAIN:PTM517 Strain Background: N2, Request a strain: please email the corresponding author Cell line (Human) Colo-205 American Type Culture Collection (Rockville, MD) Cell line (Human) MCF-7 American Type Culture Collection (Rockville, MD) Cell line (Human) MDA-MB-231 American Type Culture Collection (Rockville, MD) Cell line (Human) Hela American Type Culture Collection (Rockville, MD) Cell line (Human) A549 G. Roncador, CNIO Sequence-based reagents (Plasmid) Plasmid: pSM Cori Bargmann Lab (Rockefeller University) Sequence-based reagents (Plasmid) Plasmid: pDD162 PrU6::dpy-10_sgRNA PMID: 27467070 CRISPR/Cas9 gene editing Sequence-based reagents (Plasmid) Plasmid: pDD162 Preft3::Cas9 PMID: 27467070 CRISPR/Cas9 gene editing Sequence-based reagents (Plasmid) Plasmid: pCFJ90 Addgene http://www.wormbuilder.org /test-page/about-mossci/ Sequence-based reagents (Plasmid) Plasmid: pCFJ104 Addgene http://www.wormbuilder.org /test-page/about-mossci/ Sequence-based reagents (Plasmid) Plasmid: pCFJ151 Addgene http://www.wormbuilder.org /test-page/about-mossci/ Continued on next page Xu et al. eLife 2019;8:e48119. .. DOI: https://doi.org/10.7554/eLife.48119 23 of 38 Continued Reagent type or resource Designation Source of reference Identifiers Additional information Sequence-based reagents (Plasmid) Plasmid: pCFJ601 Addgene http://www.wormbuilder.org /test-page/about-mossci/ Antibody (mouse monoclonal) anti-HA Life Technologies Cat. No.: 326700 (1:500) Antibody (mouse monoclonal) anti-DYKDDDDK Life Technologies Cat. No.: MA191878 (1:1000) Antibody (mouse monoclonal) anti-FLAG Millipore Sigma Cat. No.: F3165 (1:1000) Antibody Horseradish peroxidaseconjugated secondary antibodies Dako Glostrup (1:10000) Peptide, recombinant protein BPTF Novus Biologicals Cat. No.: NB100-41418 Peptide, recombinant protein Vinculin Sigma Cat. No.: V9131 Sequence-based reagents (Oligonucleotide) dpy-10 (cn64) PMID: 25161212 CRISPR/Cas9 gene editing Commercial assay, kit Taqman probe: dpy-10 (kah82/kah83) Thermal: Custom TaqMan SNP Genotyping Assays PTM09 Commercial assay, kit NEB Q5 site directed mutagenesis kit NEB Cat. No.: E0554 Commercial assay, kit Next Ultra II Directional RNA Library Prep Kit NEB Cat. No.: E7760S Commercial assay, kit Zymo DNA isolation kit Zymo Cat. No.: D4071 Commercial assay, kit Zymo DNA cleanup kit Zymo Cat. No.: D4064 Commercial assay, kit ddPCR Supermix for Probes BIORAD Cat. No.: 1863010 Commercial assay, kit Droplet Generation Oils BIORAD Cat. No.: 1863005 Commercial assay, kit ddPCR Droplet Reader Oil BIORAD Cat. No.: 1863004 Commercial assay, kit VECTASHIELD antifade Mounting Medium with DAPI VECTOR Cat. No.: H-1200 Software, Algorithm edgeR PMID: 19910308 RRID:SCR_012802 Opensource: https://bioconductor.org/ packages/release/bioc /html/edgeR.html Software, Algorithm SARtools PMID: 27280887 RRID:SCR_016533 Opensource: https://github.com/ PF2-pasteur-fr/SARTools Software, Algorithm IGV PMID: 21221095 RRID:SCR_011793 https://software. broadinstitute.org/ software/igv/ Software, Algorithm Kallisto PMID: 27043002 RRID:SCR_016582 https://pachterlab. github.io/kallisto/ Software, Algorithm HISAT2 PMID: 25751142 RRID:SCR_015530 https://ccb.jhu.edu/ software/hisat2/index.shtml Continued on next page Xu et al. eLife 2019;8:e48119. .. DOI: https://doi.org/10.7554/eLife.48119 24 of 38 Continued Reagent type or resource Designation Source of reference Identifiers Additional information Software, Algorithm Samtools PMID: 19505943 RRID:SCR_002105 http://samtools. sourceforge.net/ Software, Algorithm Jalview PMID: 19151095 RRID:SCR_006459 http://www.jalview.org/ Software, Algorithm MAFFT PMID: 23329690 RRID:SCR_011811 https://mafft.cbrc.jp/ alignment/software/ Software, Algorithm IQ-Tree PMID: 25371430 RRID:SCR_017254 http://www.iqtree.org Software, Algorithm ITOL PMID: 27095192 https://itol.embl.de/ Software, Algorithm PAL2NAL PMID: 16845082

    Recombinant:

    Article Title: Evolution of Yin and Yang isoforms of a chromatin remodeling subunit precedes the creation of two genes
    Article Snippet: DOI: https://doi.org/10.7554/eLife.48119 22 of 38 Continued Reagent type or resource Designation Source of reference Identifiers Additional information Strain (C. elegans) PTM416 This paper RRID: WB-STRAIN:PTM416 Strain Background: N2, Request a strain: please email the corresponding author Strain (C. elegans) PTM417 This paper RRID: WB-STRAIN:PTM417 Strain Background: N2, Request a strain: please email the corresponding author Strain (C. elegans) PTM420 This paper RRID: WB-STRAIN:PTM420 Strain Background: N2, Request a strain: please email the corresponding author Strain (C. elegans) PTM487 This paper RRID: WB-STRAIN:PTM487 Strain Background: N2, Request a strain: please email the corresponding author Strain (C. elegans) PTM489 This paper RRID: WB-STRAIN:PTM489 Strain Background: N2, Request a strain: please email the corresponding author Strain (C. elegans) PTM512 This paper RRID: WB-STRAIN:PTM512 Strain Background: N2, Request a strain: please email the corresponding author Strain (C. elegans) PTM517 This paper RRID: WB-STRAIN:PTM517 Strain Background: N2, Request a strain: please email the corresponding author Cell line (Human) Colo-205 American Type Culture Collection (Rockville, MD) Cell line (Human) MCF-7 American Type Culture Collection (Rockville, MD) Cell line (Human) MDA-MB-231 American Type Culture Collection (Rockville, MD) Cell line (Human) Hela American Type Culture Collection (Rockville, MD) Cell line (Human) A549 G. Roncador, CNIO Sequence-based reagents (Plasmid) Plasmid: pSM Cori Bargmann Lab (Rockefeller University) Sequence-based reagents (Plasmid) Plasmid: pDD162 PrU6::dpy-10_sgRNA PMID: 27467070 CRISPR/Cas9 gene editing Sequence-based reagents (Plasmid) Plasmid: pDD162 Preft3::Cas9 PMID: 27467070 CRISPR/Cas9 gene editing Sequence-based reagents (Plasmid) Plasmid: pCFJ90 Addgene http://www.wormbuilder.org /test-page/about-mossci/ Sequence-based reagents (Plasmid) Plasmid: pCFJ104 Addgene http://www.wormbuilder.org /test-page/about-mossci/ Sequence-based reagents (Plasmid) Plasmid: pCFJ151 Addgene http://www.wormbuilder.org /test-page/about-mossci/ Continued on next page Xu et al. eLife 2019;8:e48119. .. DOI: https://doi.org/10.7554/eLife.48119 23 of 38 Continued Reagent type or resource Designation Source of reference Identifiers Additional information Sequence-based reagents (Plasmid) Plasmid: pCFJ601 Addgene http://www.wormbuilder.org /test-page/about-mossci/ Antibody (mouse monoclonal) anti-HA Life Technologies Cat. No.: 326700 (1:500) Antibody (mouse monoclonal) anti-DYKDDDDK Life Technologies Cat. No.: MA191878 (1:1000) Antibody (mouse monoclonal) anti-FLAG Millipore Sigma Cat. No.: F3165 (1:1000) Antibody Horseradish peroxidaseconjugated secondary antibodies Dako Glostrup (1:10000) Peptide, recombinant protein BPTF Novus Biologicals Cat. No.: NB100-41418 Peptide, recombinant protein Vinculin Sigma Cat. No.: V9131 Sequence-based reagents (Oligonucleotide) dpy-10 (cn64) PMID: 25161212 CRISPR/Cas9 gene editing Commercial assay, kit Taqman probe: dpy-10 (kah82/kah83) Thermal: Custom TaqMan SNP Genotyping Assays PTM09 Commercial assay, kit NEB Q5 site directed mutagenesis kit NEB Cat. No.: E0554 Commercial assay, kit Next Ultra II Directional RNA Library Prep Kit NEB Cat. No.: E7760S Commercial assay, kit Zymo DNA isolation kit Zymo Cat. No.: D4071 Commercial assay, kit Zymo DNA cleanup kit Zymo Cat. No.: D4064 Commercial assay, kit ddPCR Supermix for Probes BIORAD Cat. No.: 1863010 Commercial assay, kit Droplet Generation Oils BIORAD Cat. No.: 1863005 Commercial assay, kit ddPCR Droplet Reader Oil BIORAD Cat. No.: 1863004 Commercial assay, kit VECTASHIELD antifade Mounting Medium with DAPI VECTOR Cat. No.: H-1200 Software, Algorithm edgeR PMID: 19910308 RRID:SCR_012802 Opensource: https://bioconductor.org/ packages/release/bioc /html/edgeR.html Software, Algorithm SARtools PMID: 27280887 RRID:SCR_016533 Opensource: https://github.com/ PF2-pasteur-fr/SARTools Software, Algorithm IGV PMID: 21221095 RRID:SCR_011793 https://software. broadinstitute.org/ software/igv/ Software, Algorithm Kallisto PMID: 27043002 RRID:SCR_016582 https://pachterlab. github.io/kallisto/ Software, Algorithm HISAT2 PMID: 25751142 RRID:SCR_015530 https://ccb.jhu.edu/ software/hisat2/index.shtml Continued on next page Xu et al. eLife 2019;8:e48119. .. DOI: https://doi.org/10.7554/eLife.48119 24 of 38 Continued Reagent type or resource Designation Source of reference Identifiers Additional information Software, Algorithm Samtools PMID: 19505943 RRID:SCR_002105 http://samtools. sourceforge.net/ Software, Algorithm Jalview PMID: 19151095 RRID:SCR_006459 http://www.jalview.org/ Software, Algorithm MAFFT PMID: 23329690 RRID:SCR_011811 https://mafft.cbrc.jp/ alignment/software/ Software, Algorithm IQ-Tree PMID: 25371430 RRID:SCR_017254 http://www.iqtree.org Software, Algorithm ITOL PMID: 27095192 https://itol.embl.de/ Software, Algorithm PAL2NAL PMID: 16845082

    CRISPR:

    Article Title: Evolution of Yin and Yang isoforms of a chromatin remodeling subunit precedes the creation of two genes
    Article Snippet: DOI: https://doi.org/10.7554/eLife.48119 22 of 38 Continued Reagent type or resource Designation Source of reference Identifiers Additional information Strain (C. elegans) PTM416 This paper RRID: WB-STRAIN:PTM416 Strain Background: N2, Request a strain: please email the corresponding author Strain (C. elegans) PTM417 This paper RRID: WB-STRAIN:PTM417 Strain Background: N2, Request a strain: please email the corresponding author Strain (C. elegans) PTM420 This paper RRID: WB-STRAIN:PTM420 Strain Background: N2, Request a strain: please email the corresponding author Strain (C. elegans) PTM487 This paper RRID: WB-STRAIN:PTM487 Strain Background: N2, Request a strain: please email the corresponding author Strain (C. elegans) PTM489 This paper RRID: WB-STRAIN:PTM489 Strain Background: N2, Request a strain: please email the corresponding author Strain (C. elegans) PTM512 This paper RRID: WB-STRAIN:PTM512 Strain Background: N2, Request a strain: please email the corresponding author Strain (C. elegans) PTM517 This paper RRID: WB-STRAIN:PTM517 Strain Background: N2, Request a strain: please email the corresponding author Cell line (Human) Colo-205 American Type Culture Collection (Rockville, MD) Cell line (Human) MCF-7 American Type Culture Collection (Rockville, MD) Cell line (Human) MDA-MB-231 American Type Culture Collection (Rockville, MD) Cell line (Human) Hela American Type Culture Collection (Rockville, MD) Cell line (Human) A549 G. Roncador, CNIO Sequence-based reagents (Plasmid) Plasmid: pSM Cori Bargmann Lab (Rockefeller University) Sequence-based reagents (Plasmid) Plasmid: pDD162 PrU6::dpy-10_sgRNA PMID: 27467070 CRISPR/Cas9 gene editing Sequence-based reagents (Plasmid) Plasmid: pDD162 Preft3::Cas9 PMID: 27467070 CRISPR/Cas9 gene editing Sequence-based reagents (Plasmid) Plasmid: pCFJ90 Addgene http://www.wormbuilder.org /test-page/about-mossci/ Sequence-based reagents (Plasmid) Plasmid: pCFJ104 Addgene http://www.wormbuilder.org /test-page/about-mossci/ Sequence-based reagents (Plasmid) Plasmid: pCFJ151 Addgene http://www.wormbuilder.org /test-page/about-mossci/ Continued on next page Xu et al. eLife 2019;8:e48119. .. DOI: https://doi.org/10.7554/eLife.48119 23 of 38 Continued Reagent type or resource Designation Source of reference Identifiers Additional information Sequence-based reagents (Plasmid) Plasmid: pCFJ601 Addgene http://www.wormbuilder.org /test-page/about-mossci/ Antibody (mouse monoclonal) anti-HA Life Technologies Cat. No.: 326700 (1:500) Antibody (mouse monoclonal) anti-DYKDDDDK Life Technologies Cat. No.: MA191878 (1:1000) Antibody (mouse monoclonal) anti-FLAG Millipore Sigma Cat. No.: F3165 (1:1000) Antibody Horseradish peroxidaseconjugated secondary antibodies Dako Glostrup (1:10000) Peptide, recombinant protein BPTF Novus Biologicals Cat. No.: NB100-41418 Peptide, recombinant protein Vinculin Sigma Cat. No.: V9131 Sequence-based reagents (Oligonucleotide) dpy-10 (cn64) PMID: 25161212 CRISPR/Cas9 gene editing Commercial assay, kit Taqman probe: dpy-10 (kah82/kah83) Thermal: Custom TaqMan SNP Genotyping Assays PTM09 Commercial assay, kit NEB Q5 site directed mutagenesis kit NEB Cat. No.: E0554 Commercial assay, kit Next Ultra II Directional RNA Library Prep Kit NEB Cat. No.: E7760S Commercial assay, kit Zymo DNA isolation kit Zymo Cat. No.: D4071 Commercial assay, kit Zymo DNA cleanup kit Zymo Cat. No.: D4064 Commercial assay, kit ddPCR Supermix for Probes BIORAD Cat. No.: 1863010 Commercial assay, kit Droplet Generation Oils BIORAD Cat. No.: 1863005 Commercial assay, kit ddPCR Droplet Reader Oil BIORAD Cat. No.: 1863004 Commercial assay, kit VECTASHIELD antifade Mounting Medium with DAPI VECTOR Cat. No.: H-1200 Software, Algorithm edgeR PMID: 19910308 RRID:SCR_012802 Opensource: https://bioconductor.org/ packages/release/bioc /html/edgeR.html Software, Algorithm SARtools PMID: 27280887 RRID:SCR_016533 Opensource: https://github.com/ PF2-pasteur-fr/SARTools Software, Algorithm IGV PMID: 21221095 RRID:SCR_011793 https://software. broadinstitute.org/ software/igv/ Software, Algorithm Kallisto PMID: 27043002 RRID:SCR_016582 https://pachterlab. github.io/kallisto/ Software, Algorithm HISAT2 PMID: 25751142 RRID:SCR_015530 https://ccb.jhu.edu/ software/hisat2/index.shtml Continued on next page Xu et al. eLife 2019;8:e48119. .. DOI: https://doi.org/10.7554/eLife.48119 24 of 38 Continued Reagent type or resource Designation Source of reference Identifiers Additional information Software, Algorithm Samtools PMID: 19505943 RRID:SCR_002105 http://samtools. sourceforge.net/ Software, Algorithm Jalview PMID: 19151095 RRID:SCR_006459 http://www.jalview.org/ Software, Algorithm MAFFT PMID: 23329690 RRID:SCR_011811 https://mafft.cbrc.jp/ alignment/software/ Software, Algorithm IQ-Tree PMID: 25371430 RRID:SCR_017254 http://www.iqtree.org Software, Algorithm ITOL PMID: 27095192 https://itol.embl.de/ Software, Algorithm PAL2NAL PMID: 16845082

    Mutagenesis:

    Article Title: Evolution of Yin and Yang isoforms of a chromatin remodeling subunit precedes the creation of two genes
    Article Snippet: DOI: https://doi.org/10.7554/eLife.48119 22 of 38 Continued Reagent type or resource Designation Source of reference Identifiers Additional information Strain (C. elegans) PTM416 This paper RRID: WB-STRAIN:PTM416 Strain Background: N2, Request a strain: please email the corresponding author Strain (C. elegans) PTM417 This paper RRID: WB-STRAIN:PTM417 Strain Background: N2, Request a strain: please email the corresponding author Strain (C. elegans) PTM420 This paper RRID: WB-STRAIN:PTM420 Strain Background: N2, Request a strain: please email the corresponding author Strain (C. elegans) PTM487 This paper RRID: WB-STRAIN:PTM487 Strain Background: N2, Request a strain: please email the corresponding author Strain (C. elegans) PTM489 This paper RRID: WB-STRAIN:PTM489 Strain Background: N2, Request a strain: please email the corresponding author Strain (C. elegans) PTM512 This paper RRID: WB-STRAIN:PTM512 Strain Background: N2, Request a strain: please email the corresponding author Strain (C. elegans) PTM517 This paper RRID: WB-STRAIN:PTM517 Strain Background: N2, Request a strain: please email the corresponding author Cell line (Human) Colo-205 American Type Culture Collection (Rockville, MD) Cell line (Human) MCF-7 American Type Culture Collection (Rockville, MD) Cell line (Human) MDA-MB-231 American Type Culture Collection (Rockville, MD) Cell line (Human) Hela American Type Culture Collection (Rockville, MD) Cell line (Human) A549 G. Roncador, CNIO Sequence-based reagents (Plasmid) Plasmid: pSM Cori Bargmann Lab (Rockefeller University) Sequence-based reagents (Plasmid) Plasmid: pDD162 PrU6::dpy-10_sgRNA PMID: 27467070 CRISPR/Cas9 gene editing Sequence-based reagents (Plasmid) Plasmid: pDD162 Preft3::Cas9 PMID: 27467070 CRISPR/Cas9 gene editing Sequence-based reagents (Plasmid) Plasmid: pCFJ90 Addgene http://www.wormbuilder.org /test-page/about-mossci/ Sequence-based reagents (Plasmid) Plasmid: pCFJ104 Addgene http://www.wormbuilder.org /test-page/about-mossci/ Sequence-based reagents (Plasmid) Plasmid: pCFJ151 Addgene http://www.wormbuilder.org /test-page/about-mossci/ Continued on next page Xu et al. eLife 2019;8:e48119. .. DOI: https://doi.org/10.7554/eLife.48119 23 of 38 Continued Reagent type or resource Designation Source of reference Identifiers Additional information Sequence-based reagents (Plasmid) Plasmid: pCFJ601 Addgene http://www.wormbuilder.org /test-page/about-mossci/ Antibody (mouse monoclonal) anti-HA Life Technologies Cat. No.: 326700 (1:500) Antibody (mouse monoclonal) anti-DYKDDDDK Life Technologies Cat. No.: MA191878 (1:1000) Antibody (mouse monoclonal) anti-FLAG Millipore Sigma Cat. No.: F3165 (1:1000) Antibody Horseradish peroxidaseconjugated secondary antibodies Dako Glostrup (1:10000) Peptide, recombinant protein BPTF Novus Biologicals Cat. No.: NB100-41418 Peptide, recombinant protein Vinculin Sigma Cat. No.: V9131 Sequence-based reagents (Oligonucleotide) dpy-10 (cn64) PMID: 25161212 CRISPR/Cas9 gene editing Commercial assay, kit Taqman probe: dpy-10 (kah82/kah83) Thermal: Custom TaqMan SNP Genotyping Assays PTM09 Commercial assay, kit NEB Q5 site directed mutagenesis kit NEB Cat. No.: E0554 Commercial assay, kit Next Ultra II Directional RNA Library Prep Kit NEB Cat. No.: E7760S Commercial assay, kit Zymo DNA isolation kit Zymo Cat. No.: D4071 Commercial assay, kit Zymo DNA cleanup kit Zymo Cat. No.: D4064 Commercial assay, kit ddPCR Supermix for Probes BIORAD Cat. No.: 1863010 Commercial assay, kit Droplet Generation Oils BIORAD Cat. No.: 1863005 Commercial assay, kit ddPCR Droplet Reader Oil BIORAD Cat. No.: 1863004 Commercial assay, kit VECTASHIELD antifade Mounting Medium with DAPI VECTOR Cat. No.: H-1200 Software, Algorithm edgeR PMID: 19910308 RRID:SCR_012802 Opensource: https://bioconductor.org/ packages/release/bioc /html/edgeR.html Software, Algorithm SARtools PMID: 27280887 RRID:SCR_016533 Opensource: https://github.com/ PF2-pasteur-fr/SARTools Software, Algorithm IGV PMID: 21221095 RRID:SCR_011793 https://software. broadinstitute.org/ software/igv/ Software, Algorithm Kallisto PMID: 27043002 RRID:SCR_016582 https://pachterlab. github.io/kallisto/ Software, Algorithm HISAT2 PMID: 25751142 RRID:SCR_015530 https://ccb.jhu.edu/ software/hisat2/index.shtml Continued on next page Xu et al. eLife 2019;8:e48119. .. DOI: https://doi.org/10.7554/eLife.48119 24 of 38 Continued Reagent type or resource Designation Source of reference Identifiers Additional information Software, Algorithm Samtools PMID: 19505943 RRID:SCR_002105 http://samtools. sourceforge.net/ Software, Algorithm Jalview PMID: 19151095 RRID:SCR_006459 http://www.jalview.org/ Software, Algorithm MAFFT PMID: 23329690 RRID:SCR_011811 https://mafft.cbrc.jp/ alignment/software/ Software, Algorithm IQ-Tree PMID: 25371430 RRID:SCR_017254 http://www.iqtree.org Software, Algorithm ITOL PMID: 27095192 https://itol.embl.de/ Software, Algorithm PAL2NAL PMID: 16845082

    DNA Extraction:

    Article Title: Evolution of Yin and Yang isoforms of a chromatin remodeling subunit precedes the creation of two genes
    Article Snippet: DOI: https://doi.org/10.7554/eLife.48119 22 of 38 Continued Reagent type or resource Designation Source of reference Identifiers Additional information Strain (C. elegans) PTM416 This paper RRID: WB-STRAIN:PTM416 Strain Background: N2, Request a strain: please email the corresponding author Strain (C. elegans) PTM417 This paper RRID: WB-STRAIN:PTM417 Strain Background: N2, Request a strain: please email the corresponding author Strain (C. elegans) PTM420 This paper RRID: WB-STRAIN:PTM420 Strain Background: N2, Request a strain: please email the corresponding author Strain (C. elegans) PTM487 This paper RRID: WB-STRAIN:PTM487 Strain Background: N2, Request a strain: please email the corresponding author Strain (C. elegans) PTM489 This paper RRID: WB-STRAIN:PTM489 Strain Background: N2, Request a strain: please email the corresponding author Strain (C. elegans) PTM512 This paper RRID: WB-STRAIN:PTM512 Strain Background: N2, Request a strain: please email the corresponding author Strain (C. elegans) PTM517 This paper RRID: WB-STRAIN:PTM517 Strain Background: N2, Request a strain: please email the corresponding author Cell line (Human) Colo-205 American Type Culture Collection (Rockville, MD) Cell line (Human) MCF-7 American Type Culture Collection (Rockville, MD) Cell line (Human) MDA-MB-231 American Type Culture Collection (Rockville, MD) Cell line (Human) Hela American Type Culture Collection (Rockville, MD) Cell line (Human) A549 G. Roncador, CNIO Sequence-based reagents (Plasmid) Plasmid: pSM Cori Bargmann Lab (Rockefeller University) Sequence-based reagents (Plasmid) Plasmid: pDD162 PrU6::dpy-10_sgRNA PMID: 27467070 CRISPR/Cas9 gene editing Sequence-based reagents (Plasmid) Plasmid: pDD162 Preft3::Cas9 PMID: 27467070 CRISPR/Cas9 gene editing Sequence-based reagents (Plasmid) Plasmid: pCFJ90 Addgene http://www.wormbuilder.org /test-page/about-mossci/ Sequence-based reagents (Plasmid) Plasmid: pCFJ104 Addgene http://www.wormbuilder.org /test-page/about-mossci/ Sequence-based reagents (Plasmid) Plasmid: pCFJ151 Addgene http://www.wormbuilder.org /test-page/about-mossci/ Continued on next page Xu et al. eLife 2019;8:e48119. .. DOI: https://doi.org/10.7554/eLife.48119 23 of 38 Continued Reagent type or resource Designation Source of reference Identifiers Additional information Sequence-based reagents (Plasmid) Plasmid: pCFJ601 Addgene http://www.wormbuilder.org /test-page/about-mossci/ Antibody (mouse monoclonal) anti-HA Life Technologies Cat. No.: 326700 (1:500) Antibody (mouse monoclonal) anti-DYKDDDDK Life Technologies Cat. No.: MA191878 (1:1000) Antibody (mouse monoclonal) anti-FLAG Millipore Sigma Cat. No.: F3165 (1:1000) Antibody Horseradish peroxidaseconjugated secondary antibodies Dako Glostrup (1:10000) Peptide, recombinant protein BPTF Novus Biologicals Cat. No.: NB100-41418 Peptide, recombinant protein Vinculin Sigma Cat. No.: V9131 Sequence-based reagents (Oligonucleotide) dpy-10 (cn64) PMID: 25161212 CRISPR/Cas9 gene editing Commercial assay, kit Taqman probe: dpy-10 (kah82/kah83) Thermal: Custom TaqMan SNP Genotyping Assays PTM09 Commercial assay, kit NEB Q5 site directed mutagenesis kit NEB Cat. No.: E0554 Commercial assay, kit Next Ultra II Directional RNA Library Prep Kit NEB Cat. No.: E7760S Commercial assay, kit Zymo DNA isolation kit Zymo Cat. No.: D4071 Commercial assay, kit Zymo DNA cleanup kit Zymo Cat. No.: D4064 Commercial assay, kit ddPCR Supermix for Probes BIORAD Cat. No.: 1863010 Commercial assay, kit Droplet Generation Oils BIORAD Cat. No.: 1863005 Commercial assay, kit ddPCR Droplet Reader Oil BIORAD Cat. No.: 1863004 Commercial assay, kit VECTASHIELD antifade Mounting Medium with DAPI VECTOR Cat. No.: H-1200 Software, Algorithm edgeR PMID: 19910308 RRID:SCR_012802 Opensource: https://bioconductor.org/ packages/release/bioc /html/edgeR.html Software, Algorithm SARtools PMID: 27280887 RRID:SCR_016533 Opensource: https://github.com/ PF2-pasteur-fr/SARTools Software, Algorithm IGV PMID: 21221095 RRID:SCR_011793 https://software. broadinstitute.org/ software/igv/ Software, Algorithm Kallisto PMID: 27043002 RRID:SCR_016582 https://pachterlab. github.io/kallisto/ Software, Algorithm HISAT2 PMID: 25751142 RRID:SCR_015530 https://ccb.jhu.edu/ software/hisat2/index.shtml Continued on next page Xu et al. eLife 2019;8:e48119. .. DOI: https://doi.org/10.7554/eLife.48119 24 of 38 Continued Reagent type or resource Designation Source of reference Identifiers Additional information Software, Algorithm Samtools PMID: 19505943 RRID:SCR_002105 http://samtools. sourceforge.net/ Software, Algorithm Jalview PMID: 19151095 RRID:SCR_006459 http://www.jalview.org/ Software, Algorithm MAFFT PMID: 23329690 RRID:SCR_011811 https://mafft.cbrc.jp/ alignment/software/ Software, Algorithm IQ-Tree PMID: 25371430 RRID:SCR_017254 http://www.iqtree.org Software, Algorithm ITOL PMID: 27095192 https://itol.embl.de/ Software, Algorithm PAL2NAL PMID: 16845082

    Software:

    Article Title: Evolution of Yin and Yang isoforms of a chromatin remodeling subunit precedes the creation of two genes
    Article Snippet: DOI: https://doi.org/10.7554/eLife.48119 22 of 38 Continued Reagent type or resource Designation Source of reference Identifiers Additional information Strain (C. elegans) PTM416 This paper RRID: WB-STRAIN:PTM416 Strain Background: N2, Request a strain: please email the corresponding author Strain (C. elegans) PTM417 This paper RRID: WB-STRAIN:PTM417 Strain Background: N2, Request a strain: please email the corresponding author Strain (C. elegans) PTM420 This paper RRID: WB-STRAIN:PTM420 Strain Background: N2, Request a strain: please email the corresponding author Strain (C. elegans) PTM487 This paper RRID: WB-STRAIN:PTM487 Strain Background: N2, Request a strain: please email the corresponding author Strain (C. elegans) PTM489 This paper RRID: WB-STRAIN:PTM489 Strain Background: N2, Request a strain: please email the corresponding author Strain (C. elegans) PTM512 This paper RRID: WB-STRAIN:PTM512 Strain Background: N2, Request a strain: please email the corresponding author Strain (C. elegans) PTM517 This paper RRID: WB-STRAIN:PTM517 Strain Background: N2, Request a strain: please email the corresponding author Cell line (Human) Colo-205 American Type Culture Collection (Rockville, MD) Cell line (Human) MCF-7 American Type Culture Collection (Rockville, MD) Cell line (Human) MDA-MB-231 American Type Culture Collection (Rockville, MD) Cell line (Human) Hela American Type Culture Collection (Rockville, MD) Cell line (Human) A549 G. Roncador, CNIO Sequence-based reagents (Plasmid) Plasmid: pSM Cori Bargmann Lab (Rockefeller University) Sequence-based reagents (Plasmid) Plasmid: pDD162 PrU6::dpy-10_sgRNA PMID: 27467070 CRISPR/Cas9 gene editing Sequence-based reagents (Plasmid) Plasmid: pDD162 Preft3::Cas9 PMID: 27467070 CRISPR/Cas9 gene editing Sequence-based reagents (Plasmid) Plasmid: pCFJ90 Addgene http://www.wormbuilder.org /test-page/about-mossci/ Sequence-based reagents (Plasmid) Plasmid: pCFJ104 Addgene http://www.wormbuilder.org /test-page/about-mossci/ Sequence-based reagents (Plasmid) Plasmid: pCFJ151 Addgene http://www.wormbuilder.org /test-page/about-mossci/ Continued on next page Xu et al. eLife 2019;8:e48119. .. DOI: https://doi.org/10.7554/eLife.48119 23 of 38 Continued Reagent type or resource Designation Source of reference Identifiers Additional information Sequence-based reagents (Plasmid) Plasmid: pCFJ601 Addgene http://www.wormbuilder.org /test-page/about-mossci/ Antibody (mouse monoclonal) anti-HA Life Technologies Cat. No.: 326700 (1:500) Antibody (mouse monoclonal) anti-DYKDDDDK Life Technologies Cat. No.: MA191878 (1:1000) Antibody (mouse monoclonal) anti-FLAG Millipore Sigma Cat. No.: F3165 (1:1000) Antibody Horseradish peroxidaseconjugated secondary antibodies Dako Glostrup (1:10000) Peptide, recombinant protein BPTF Novus Biologicals Cat. No.: NB100-41418 Peptide, recombinant protein Vinculin Sigma Cat. No.: V9131 Sequence-based reagents (Oligonucleotide) dpy-10 (cn64) PMID: 25161212 CRISPR/Cas9 gene editing Commercial assay, kit Taqman probe: dpy-10 (kah82/kah83) Thermal: Custom TaqMan SNP Genotyping Assays PTM09 Commercial assay, kit NEB Q5 site directed mutagenesis kit NEB Cat. No.: E0554 Commercial assay, kit Next Ultra II Directional RNA Library Prep Kit NEB Cat. No.: E7760S Commercial assay, kit Zymo DNA isolation kit Zymo Cat. No.: D4071 Commercial assay, kit Zymo DNA cleanup kit Zymo Cat. No.: D4064 Commercial assay, kit ddPCR Supermix for Probes BIORAD Cat. No.: 1863010 Commercial assay, kit Droplet Generation Oils BIORAD Cat. No.: 1863005 Commercial assay, kit ddPCR Droplet Reader Oil BIORAD Cat. No.: 1863004 Commercial assay, kit VECTASHIELD antifade Mounting Medium with DAPI VECTOR Cat. No.: H-1200 Software, Algorithm edgeR PMID: 19910308 RRID:SCR_012802 Opensource: https://bioconductor.org/ packages/release/bioc /html/edgeR.html Software, Algorithm SARtools PMID: 27280887 RRID:SCR_016533 Opensource: https://github.com/ PF2-pasteur-fr/SARTools Software, Algorithm IGV PMID: 21221095 RRID:SCR_011793 https://software. broadinstitute.org/ software/igv/ Software, Algorithm Kallisto PMID: 27043002 RRID:SCR_016582 https://pachterlab. github.io/kallisto/ Software, Algorithm HISAT2 PMID: 25751142 RRID:SCR_015530 https://ccb.jhu.edu/ software/hisat2/index.shtml Continued on next page Xu et al. eLife 2019;8:e48119. .. DOI: https://doi.org/10.7554/eLife.48119 24 of 38 Continued Reagent type or resource Designation Source of reference Identifiers Additional information Software, Algorithm Samtools PMID: 19505943 RRID:SCR_002105 http://samtools. sourceforge.net/ Software, Algorithm Jalview PMID: 19151095 RRID:SCR_006459 http://www.jalview.org/ Software, Algorithm MAFFT PMID: 23329690 RRID:SCR_011811 https://mafft.cbrc.jp/ alignment/software/ Software, Algorithm IQ-Tree PMID: 25371430 RRID:SCR_017254 http://www.iqtree.org Software, Algorithm ITOL PMID: 27095192 https://itol.embl.de/ Software, Algorithm PAL2NAL PMID: 16845082



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